Isolation of a novel cell-attachment and spreading-promoting protein from human serum.
نویسندگان
چکیده
A protein with potent cell-attachment and spreading-promoting activity was isolated from fibronectin-free human serum. The purification steps included affinity chromatography on heparin-agarose and preparative isoelectric focusing. The purified protein was homogeneous as judged from dodecyl sulphate/polyacrylamide-gel electrophoresis. It had an isoelectric point of 5.0 and an Mr of 52 000. The protein promoted the spreading of Chinese-hamster ovary cells to plastic in a manner similar to that observed with fibronectin.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 227 2 شماره
صفحات -
تاریخ انتشار 1985